Machine-translated by Google. Questions are authored in English.
Top

Discussion

Why do proteins absorb near 280 nanometres?

  • A.Disulphide bridges absorb strongly there
  • B.Aromatic side chains provide the chromophores
  • C.The peptide backbone absorbs at that wavelength
  • D.Bound metal ions absorb in that region

Answer: B

Tryptophan and tyrosine, with their aromatic rings, are the principal absorbers near 280 nanometres, which is why the band's size depends on a protein's composition and why an absorptivity must be known or estimated for each one. The backbone absorbs far shorter, around 190 to 220, and metals and disulphides contribute little. That a routine concentration measurement depends on the particular amino acids present is a limitation worth knowing before the number is trusted.

No comment is present. Be the first to comment.
Loading…

Post your comment

Please log in or register to post a comment.