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The test(s) used for the assay of cholera toxin is/are

  • A.Radioimmunoassay
  • B.Enzyme-linked immunosorbent assay
  • C.Morphological changes in Chinese hamster ovary cells
  • D.All of the above

Answer: D

Correct answer: All of the above

All three detect the toxin, and they answer slightly different questions. Radioimmunoassay and ELISA measure it immunologically and are quantitative; the Chinese hamster ovary cell assay detects biological activity, the cells elongating characteristically in response. A biological assay confirms that what has been detected is functional toxin rather than an inactive fragment.

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