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Which of the followings can be used to differentiate between classical and El Tor biotypes of Vibrio cholerae?

  • A.Sensitivity to Mukerjee's group IV phage
  • B.Agglutination of fowl RBCs
  • C.Sensitivity to polymyxin B
  • D.All of these

Answer: D

Correct answer: All of these

The three tests separate the biotypes in three different ways. The classical biotype is sensitive to Mukerjee’s group IV phage and to polymyxin B and does not agglutinate fowl red cells; El Tor is resistant to both and does agglutinate them. The Voges-Proskauer reaction and haemolysis are used alongside them, and no single test is relied on alone.

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