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A plaque in a virus-infected cell monolayer represents

  • A.a colony of virions that has grown on the surface of agar
  • B.an area where antibody has precipitated
  • C.a patch of uninfected cells resistant to the virus
  • D.a zone of cells destroyed by virus spreading from one particle

Answer: D

One infectious particle infects a cell, its progeny infect neighbours, and the resulting cleared zone becomes visible against the intact monolayer. Because each plaque traces back to a single particle, counting them gives the titre in plaque-forming units.

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