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Which of these is used when a virus cannot be isolated in cell culture?

  • A.Incubation in a cell-free extract
  • B.Inoculation into suckling mice
  • C.Subculture onto blood agar
  • D.Growth in nutrient broth

Answer: B

Isolation occasionally requires inoculation into animals: suckling mice, rabbits, guinea pigs, hamsters or primates. The choice depends on which host supports that virus, and the practice persists for agents cell lines will not sustain.

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