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Discussion

A plaque assay measures

  • A.the temperature at which a virus is inactivated
  • B.intercellular spread and cytopathic effect
  • C.the total mass of viral protein present
  • D.the number of physical particles whether infectious or not

Answer: B

Each plaque records that a virus was able to enter a cell, replicate, spread to its neighbours and damage them, so the count reflects infectivity rather than particle number. Many particles in a preparation are non-infectious and are invisible to this assay.

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