Discussion
A plaque appears in a monolayer because
Answer: A
Correct answer: infected cells have died, leaving a clear area
A plaque is a hole in the monolayer. One infectious particle infects one cell, the progeny spread to the neighbours under a semi-solid overlay that prevents diffusion, and the successive rounds of killing leave a clear patch against the stained sheet of surviving cells. Because each plaque traces back to a single particle, counting plaques counts infectious units.
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