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MH SET 2019: 23 June, Paper II, Life Sciences

  • Date: 23 June 2019
  • Paper code: JUN-34219
  • Series: A
  • Language: Bilingual
  • 24 pages
MH SET 2019 question paper, 23 June 2019, Paper II, Life Sciences, page 1 of 24
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Test Booklet Code & Serial No.
                                                                                                                     A
                                                             LIFE SCIENCE
Signature and Name of Invigilator                                                                 Seat No.
1. (Signature) .........................................                                                   (In figures as in Admit Card)
      (Name) ................................................                     Seat No. ..............................................................
2. (Signature) .........................................                                                    (In words)

      (Name) ................................................                       OMR Sheet No.
JUN - 34219                                                                                          (To be filled by the Candidate)
Time Allowed : 2 Hours]                                                                                [Maximum Marks : 200
Number of Pages in this Booklet : 24                                                    Number of Questions in this Booklet : 100
                      Instructions for the Candidates
1.         Write your Seat No. and OMR Sheet No. in the space provided             1.
           on the top of this page.
2.         This paper consists of 100 objective type questions. Each question
           will carry two marks. All questions of Paper II will be compulsory.     2.
3.         At the commencement of examination, the question booklet
           will be given to the student. In the first 5 minutes, you are           3.
           requested to open the booklet and compulsorily examine it as
           follows :
           (i)      To have access to the Question Booklet, tear off the
                    paper seal on the edge of this cover page. Do not accept               (i)
                    a booklet without sticker-seal or open booklet.
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                    the booklet with the information printed on the cover                  (ii)
                    page. Faulty booklets due to missing pages/questions
                    or questions repeated or not in serial order or any
                    other discrepancy should not be accepted and correct
                    booklet should be obtained from the invigilator within
                    the period of 5 minutes. Afterwards, neither the Question
                    Booklet will be replaced nor any extra time will be
                    given. The same may please be noted.
           (iii)    After this verification is over, the OMR Sheet Number
                                                                                           (iii)
                    should be entered on this Test Booklet.
4.         Each question has four alternative responses marked (A), (B),
           (C) and (D). You have to darken the circle as indicated below on        4.                      (A), (B), (C)    (D)
           the correct response against each item.
           Example : where (C) is the correct response.

                              A        B                 D                                           (C)
5.         Your responses to the items are to be indicated in the OMR                                      A        B               D
           Sheet given inside the Booklet only. If you mark at any place
           other than in the circle in the OMR Sheet, it will not be evaluated.    5.
6.         Read instructions given inside carefully.
7.         Rough Work is to be done at the end of this booklet.                    6.
8.         If you write your Name, Seat Number, Phone Number or put                7.
           any mark on any part of the OMR Sheet, except for the space
                                                                                   8.
           allotted for the relevant entries, which may disclose your
           identity, or use abusive language or employ any other unfair
           means, you will render yourself liable to disqualification.
9.         You have to return original OMR Sheet to the invigilator at the
           end of the examination compulsorily and must not carry it with          9.
           you outside the Examination Hall. You are, however, allowed
           to carry the Test Booklet and duplicate copy of OMR Sheet on
           conclusion of examination.
                                                                                   10.
10.        Use only Blue/Black Ball point pen.
                                                                                   11.
11.        Use of any calculator or log table, etc., is prohibited.
12.        There is no negative marking for incorrect answers.                     12.
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JUN - 34219/II—A




2
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JUN - 34219/II—A

                                   Life Science
                                     Paper II
Time Allowed : 120 Minutes]                                [Maximum Marks : 200

Note : This Paper contains Hundred (100) multiple choice questions. Each question

          carrying Two (2) marks. Attempt All questions.


1.   Archegonia are absent in the ovule       3.   Which of the following is NOT

                                                   caused by deficiency of mineral
     of ...................... .
                                                   nutrition ?
     (A) Cycas
                                                   (A) Etiolation
     (B) Taxus
                                                   (B) Shortening of internode
     (C) Pinus
                                                   (C) Necrosis

     (D) Gnetum                                    (D) Chlorosis

2.   Membranes of which one of the            4.   Rous sarcoma virus, which induces

     following pairs are contiguous ?              the formation of sarcomas in

                                                   chickens. Which of the following is
     (A) ER and Golgi bodies
                                                   the product of oncogene V-src ?
     (B) Nucleus and ER
                                                   (A) Epidermal Growth Factor (EGF)

     (C) Golgi bodies and plasma                   (B) Tyrosine Kinase
            membrane
                                                   (C) Tyrosinase

     (D) Golgi bodies and lysosomes                (D) Serine Kinase

                                          3                                    [P.T.O.
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5.   Hemophilia and colour blindness           7.   Maple syrup urine disease, one of the

     are :                                          inherited metabolic disorder is

                                                    caused because of blockage of
     (A) Autosomal dominant inheritance
                                                    oxidative decarboxylation of   -keto
         patterns
                                                    acids derived from valine, leucine
     (B) Y-linked recessive inheritance
                                                    and isoleucine. Which of the
     (C) X-linked recessive inheritance
                                                    following enzyme is missing or
     (D) Y- and X-linked dominant                   defective in the patient ?
         inheritance respectively
                                                    (A) Homogentisate reductase
6.   Which    one   of   the   following
                                                    (B) Branched chain dehydrogenase
     statements is correct ?
                                                    (C) Oxaloacetate decarboxylase
     (A) Chromosomes separate in
                                                    (D) Acetoacetate carboxylase
         meiosis I and chromatids
                                               8.   Which one of the following sequences
         separate in meiosis II
                                                    functions as a signal for N-linked
     (B) Chromosomes separate in
                                                    glycosylation in rough endoplasmic
         meiosis II and chromatids
                                                    reticulum ?
         separate in meiosis I
                                                    (A) Asn-X-Ser and Asn-X-Thr
     (C) Chromosomes separate in both
                                                    (B) Asn-X-Ser and Asn-X-Pro
         meiosis I and meiosis II

                                                    (C) Asn-X-Thr and Asn-X-Gly
     (D) Chromatids separate in both,

         meiosis I and meiosis II                   (D) Asn-X-Gly and Asn-X-Pro

                                           4
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9.   Oxysomes for F0-F1 particles are          11. By what process does Thorium-230

     present on :                                  decay to radium-226 ?

     (A) Thylakoids
                                                   (A) Gamma emission

     (B) Outer mitochondrial membrane
                                                   (B) Alpha emission
     (C) Outer chloroplast membrane
                                                   (C) Beta emission
     (D) Inner mitochondrial membrane
                                                   (D) Electron capture
10. What is the sequence of cell
                                               12. Which of the following is the most
     organelles getting separated during

     cell fractionation by differential            suitable mode to study protein

     centrifugation ?                              conformational changes and to probe

     (A) Nucleus, mitochondria, micro-             the location of active sites and

         somes, cytosol                            coenzymes in fluorescence spectro-

     (B) Cytosol, nucleus, microsomes,             scopy ?

         mitochondria
                                                   (A) Quenching

     (C) Microsomes, mitochondria,
                                                   (B) Quantum yield
         cytosol, nucleus
                                                   (C) Intrinsic fluorescence
     (D) Cytosol, microsomes, mito-
                                                   (D) Extrinsic fluorescence
         chondria, nucleus

                                           5                                    [P.T.O.
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13. Which of the following is NOT a             16. Which one of the following is
    method used in mass spectroscopy                produced during water stress and
    for introducing the sample without              that brings stomatal closure ?
    thermal decomposition ?
                                                    (A) Ethylene
    (A) MALDI
                                                    (B) Abscisic acid
    (B) Electrospray
                                                    (C) Ferulic acid
    (C) Plasma desorption
                                                    (D) Coumarin
    (D) Pyrolysis
                                                17. Which of the following enzymes is
14. In polyacrylamide gel electro-
                                                    not a part of fatty acid synthetase
    phoresis, the breakdown of disulfide
                                                    complex ?
    linkages in protein is carried out by
    using :                                         (A) Enoyl reductase

    (A) TEMED                                       (B)    -hydroxy acyl CoA dehydro-
                                                          genase
    (B) Bisacrylamide
                                                    (C)    -keto acyl reductase
    (C)    -mercaptoethanol
                                                    (D) Acetyl transacylase
    (D) SDS

15. Helianthus annuus flowers track             18. Reducing power required for

    the sun. This phenomenon is known               biosynthesis of fatty acids in liver

    as :                                            is provided by :

    (A) Phototropism                                (A) TCA cycle

    (B) Photoperiodism                              (B)    -oxidation of fatty acid

    (C) Positive heliotropism                       (C) Hexose monophosphate shunt

    (D) Negative heliotropism                       (D) Glycogenolysis

                                            6
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19. Starting with a fertilized egg              22. Somatostatins and opioids are
                                                    examples of :
    (zygote), a series of sequential five
    cell divisions would produce an early           (A) Purine neurotransmitters

    embryo with how many cells ?                    (B) Peptide neurotransmitters

    (A) 8                                           (C) Amino acid neurotransmitters

    (B) 16                                          (D) Mono amine neurotransmitters

    (C) 32                                      23. Nucellar polyembryony is common
                                                    in species of :
    (D) 64
                                                    (A) Citrus
20. If the Golgi apparatus is associated
                                                    (B) Citronella
    with secretion, we would expect it
    most abundantly in :                            (C) Croton

    (A) Muscle                                      (D) Crotallaria

                                                24. The conformation of the polypeptide
    (B) Blood
                                                    chain is determined by the torsion
    (C) Gland
                                                    or rotation angles around C –N and
    (D) Bone                                        C –C bonds of each amino acid
                                                    participating in it. Which of the
21. Which one of the following is NOT
                                                    following pairs of amino acids is
    a function of kidney ?
                                                    conformationally the most and the
    (A) Thermoregulation                            least restricted amino acids ?

    (B) Coordinated muscle movement                 (A) Glycine and Proline

    (C) Blood pressure control                      (B) Proline and Alanine

    (D) Maintaining ionic balance and               (C) Alanine and Proline

         pH of the blood                            (D) Proline and Glycine

                                            7                                   [P.T.O.
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25. Prostaglandin H2 synthase a key
    enzyme in the synthesis of prosta-           27. In a normal kidney Bowman’s
    glandins from linear fatty acid
    has two types of catalytic activities.            capsule, the blood is filtered and the
    They are :
                                                      filtrate contains :
      (A) Cyclooxygenase and Reductase
      (B) Monooxygenase and Reductase
                                                      (A) RBC, haemoglobin, water,
      (C) Monooxygenase and Peroxidase
      (D) Cyclooxygenase and Peroxidase                   glucose and small molecular

26.       Glucose-6-Phosphate
                                                          weight proteins


          Fructose-6-Phosphate                        (B) Water and glucose


                                                      (C) Water, glucose and large

                                                          molecular weight proteins


       Fructose-1, 6-bisphosphate                     (D) Only water


                                                 28. In tender coconut, initially the endo-
Glyceraldehyde-3-Phosphate +
            Dihydroxyacetone Phosphate                sperm is :
      Such a set of opposing reaction
      shown as (A) and (B) in above figure            (A) Free nuclear
      is an example of :
                                                      (B) Cellular
      (A) Reversible reaction

      (B) Substrate cycle                             (C) Helobial
      (C) Product cycle
                                                      (D) Both cellular and helobial
      (D) Enzyme cycle
                                             8
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29. The lymphatic system has the                31. Liver is the principal site of
                                                    synthesis of :
    following functions except :
                                                    (A) Plasma albumin
    (A) It maintains the balance of fluid
                                                    (B) Plasma globulin
        between the blood and tissues,
                                                    (C) Vitamin B12
        known as fluid homeostasis.
                                                    (D) Vitamin C
    (B) It forms part of the immine
                                                32. Which one of the following mutagens
        system and helps defend against
                                                    can add alkyl group to guanine
        bacteria and other pathogens.               resulting in direct mispairing with
                                                    thymine ?
    (C) It facilitates absorption of fats
                                                    (A) 5-bromo uracil
        and fat-soluble nutrients in the
                                                    (B) UV-rays
        digestive system.
                                                    (C) Acridine orange
    (D) It facilitates exchange of gases
                                                    (D) EMS
        in tissues.
                                                33. Hox genes are critical for the proper
30. Which is the source of foodstuff                placement of segment structures of
    ‘Nori’ ?                                        animals during early embryonic
                                                    development except :
    (A) Porphyra
                                                    (A) Different vertebrae of humans
    (B) Polytrichum
                                                    (B) Caecum in humans
    (C) Potamogeton
                                                    (C) Antennae in fruit flies

    (D) Pteridium                                   (D) Wings in fruit flies

                                            9                                     [P.T.O.
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34. Mutation in which one of the                    37. In the ATP synthase of mitochondria
     following proteins will inhibit
                                                        .................. subunit has the ATP/ADP
     recognition of mismatched base pairs
     during DNA repair ?                                binding site.

     (A) Mut H                                          (A) Alpha

     (B) Mut S                                          (B) Beta
     (C) Uvr D
                                                        (C) Gamma
     (D) Mut L
                                                        (D) Delta
35. ..................... is involved in the
     specification of germ cells in all             38. In electron microscopy the fixative
     animals studied.
                                                        used for plant materials is :
     (A) Sry
                                                        (A) Alcohol
     (B) Sox 9
                                                        (B) Acetic alcohol
     (C) Wnt 4

     (D) Vasa                                           (C) Glutaraldehyde

36. The lagging strand of DNA is                        (D) Formaldehyde
     replicated in short pieces because :
                                                    39. Continuous activity of cambium in
     (A) Of limitation of space
                                                        the stem leads to :
     (B) Otherwise the helix will become
          distorted                                     (A) Diffuse porous wood

     (C) DNA polymerase can synthesize                  (B) Ring porous wood
          in one direction only
                                                        (C) Fibrous wood
     (D) To make proof-reading of code
          easier                                        (D) Semi ring porous wood

                                               10
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40. In Leishmania it was shown that             42. If red and white seed colour shows

    the minicircles :                               a ratio of 1 : 4 : 6 : 4 : 1 in F2

    (A) Encode guide RNA (gRNA)                     generation, it is due to :

        molecules involved in the RNA
                                                    (A) Duplicate genes
        editing of maxicircle cryptogene
                                                    (B) Polygenic trait controlled by
    (B) Encode guide RNA (gRNA)
                                                        three genes
        molecules involved in the RNA
                                                    (C) Epistatic genes
        editing of minicircle cryptogene

                                                    (D) Polygenic trait controlled by two
    (C) Encode micro RNA
                                                        genes
    (D) Encode noncoding RNA
                                                43. The process of neoplastic cells
41. With reference to plants, micro-
                                                    moving through the circulatory
    propagation in vitro means :
                                                    system and becoming lodged in a
    (A) Propagation of tiny plants
                                                    vessel causing obstruction is referred
    (B) Production of a large number of
                                                    to as :
        progeny plants
                                                    (A) Anaplasia
    (C) Production      of   plants   at
                                                    (B) Neoplasia
        microlevel

    (D) Production of plants from                   (C) Thrombosis

        microspores                                 (D) Embolism
                                           11                                     [P.T.O.
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44. Double lines in pedigree analysis          47. A   small    organic   non-protein
    shows :                                        molecule that carries chemical

    (A) Sex unspecific                             groups between enzymes is :

    (B) Unaffected offspring                       (A) Cofactor

    (C) Marriages between related                  (B) Coenzyme
        individuals
                                                   (C) Catalyst
    (D) Marriages between unrelated
                                                   (D) Substrate
        individuals
                                               48. The key element in the magnetic
45. Down’s syndrome is caused due to
                                                   sensor system of pigeons and
    aberration in :
                                                   migrating birds is :
    (A) Chromosome 2
                                                   (A) Columella
    (B) Chromosome 20
                                                   (B) Cochlea
    (C) Chromosome 21
                                                   (C) Lagena
    (D) X-Chromosome
                                                   (D) Extracolumella
46. The enzyme useful in conversion of
                                               49. X-rays induce mutagenic changes
    penicillin G to 6-aminopenicillonic
    acid is :                                      by :

    (A) Penicillin acylase                         (A) Transitions

    (B) Penicillinase                              (B) Frame shifting

    (C) Carboxypeptidase                           (C) Transversions

    (D) Aminopeptidase                             (D) Chromosomal breakage

                                          12
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50. Which of the following sn RNAs pair        53. The ..................... of the influenza-

    to form the catalytic active site               enveloped virus appear to be

    during pre-mRNA splicing ?                      involved in attachment to the host
                                                    cell receptor site.
    (A) U6-U2
                                                    (A) Pili
    (B) U1-U2
                                                    (B) Fimbriae
    (C) U6-U4
                                                    (C) Haemagglutinin
    (D) U5-U4
                                                    (D) Neuraminidase
51. The progeny of a single homozygous
                                               54. Eating meat from cattle with bovine
    self-pollinated plant is called                 spongiform encephalitis can cause a
    as ....................... .                    variant of ................. in humans.

    (A) Inbred line                                 (A) Kuru

    (B) Isogenic line                               (B) Fatal familiar insomnia

    (C) Pureline                                    (C) Creutzfeldt-Jakob disease

                                                    (D) Geistmann-Straussler-Scheinker
    (D) Near-isogenic line
                                                           syndrome
52. Female gametophyte in Gnetum at
                                               55. Saffron is produced from the flowers
    later stage is :
                                                    of ...................... .
    (A) Uninucleate
                                                    (A) Mesua ferrea
    (B) Binucleate
                                                    (B) Crocus sativus
    (C) Eight nucleate                              (C) Nelumbo nucifera

    (D) Multinucleate                               (D) Mammea suriga

                                          13                                                [P.T.O.
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56. Lyme disease is spread by :                    59. Chromosomal elimination is one of

                                                       the strategy to develop .................. .
    (A) Bed bugs
                                                       (A) Polyploids
    (B) Leech
                                                       (B) Amphidiploid
    (C) Mosquito
                                                       (C) Haploid
    (D) Ticks
                                                       (D) Autopolyploid
57. Which of the following pairs is NOT
                                                   60. Which component of prokaryotic
    correct ?
                                                       RNA polymerase facilitates the
    (A) C3 plants - Maize
                                                       recognition of promoter sequences ?
    (B) C4 plants - Kranz anatomy
                                                       (A)     subunit

    (C) Calvin cycle - PGA
                                                       (B)     subunit

    (D) Hatch and slack cycle - Oxaloacetic
                                                       (C)     subunit
         acid
                                                       (D)     subunit
58. Delay in senescence in the plants is
                                                   61. An appropriate term for human
    caused by :
                                                       resident flora is :

    (A) IBA
                                                       (A) Commensals

    (B) IAA                                            (B) Parasites

    (C) Cytokinin                                      (C) Pathogens

    (D) Gibberellin                                    (D) Mutualists

                                              14
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62. Transplantation of stem cell                  65. Methyl transferases of restriction

    populations from genetically identical            modification systems seem to have

    donor to the recipient is said to be :            evolved by :

    (A) Autologus                                     (A) Convergent evolution

    (B) Syngeneic                                     (B) Divergent evolution

                                                      (C) Mix    of   convergent        and
    (C) Allogeneic
                                                           divergent evolution
    (D) Xenogeneic
                                                      (D) Natural evolution
63. For thorough mixing of medium and
                                                  66. Which of the following is NOT a
    inoculum the part of the fermentor
                                                      premating mechanism of isolation ?
    useful is :
                                                      (A) Temporal isolation
    (A) Shaft
                                                      (B) Zygotic mortality
    (B) Headspace
                                                      (C) Behavioural isolation
    (C) Impeller
                                                      (D) Mechanical isolation
    (D) Sparger
                                                  67. A clade is a set of species :
64. Peptide antigen assemble with
                                                      (A) Derived     from    a     common
    class 1 MHC aided by :
                                                           ancestor
    (A) Chaperone molecules
                                                      (B) Derived from two ancestors
    (B) Immunoglobulins
                                                      (C) Derived from multiple ancestors
    (C) T-cell receptor
                                                      (D) Inhabiting     a        particular
    (D) Cytokines                                          environment

                                             15                                       [P.T.O.
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68. Center of origin of coffee is :             70. The zone at the edge of lake, sea or

    (A) Brazil                                      ocean which is alternatively exposed

    (B) Peru                                        to air and immersed in water is

    (C) Moluccas                                    called ........................ .

    (D) Ethiopia
                                                    (A) Pelagic zone

69. Difference between Allopatric and
                                                    (B) Benthic zone
    Sympatric speciation is with respect

    to :                                            (C) Lentic zone


    (i)    Attainment of reproductive               (D) Littoral zone

           isolation
                                                71. What is the ultimate function of any
    (ii) Geographical separation
                                                    ecosystem ?
    (iii) Overlap in their distribution
                                                    (A) Survival of species
    (A) Only (i)

                                                    (B) Balance of natural system
    (B) Only (ii)


    (C) Only (iii)                                  (C) Flow of energy


    (D) (i), (ii) and (iii)                         (D) Biological control

                                           16
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72. The appearance of lichens and moss            75. The population of asiatic lion is
    where there were previously no
                                                      small. Therefore, there is greater
    living things is an indication of the
                                                      chance of :
    start of :

    (A) Intraspecific competition                     (A) Gene flow

    (B) Environmental resistance                      (B) Genetic drift

    (C) Primary succession
                                                      (C) Mutation
    (D) Resource partitioning
                                                      (D) Selection
73. If one wishes to compare the means
    of two independent samples, the               76. Which of the statements regarding

    appropriate test statistics would be :            DNA replication is correct ?

    (A) F-test                                        (A) The       leading   strand     is
    (B) Student t-test                                    synthesized discontinuously

    (C) Chi-square                                        from multiple primers.

    (D) Correlation coefficient analysis
                                                      (B) The polymerase enzyme caps
74. On an average, which ecosystem has
                                                          the 5 end of the nascent DNA
    the lowest net primary productivity
                                                          strand
    per unit area ?

    (A) An open ocean                                 (C) Lagging strand helicases are

    (B) An estuary                                        composed of 5 identical sub-units

    (C) A freshwater lake                             (D) Synthesis of the leading strand

    (D) A coral reef                                      requires one RNA primer

                                             17                                    [P.T.O.
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77. The transposase gene encodes an             79. The phosphorus cycle is unusual in

    enzyme that :                                   that it is entirely :


    (A) Facilitates general recom-                  (A) Within the aquatic ecosystem

         bination                                   (B) Within the terrestrial ecosystem

    (B) Facilitates viral replication               (C) Sedimentary

         within a genome                            (D) Gaseous

    (C) Facilitates        site-specific        80. Which one of the following is a

         integration of transposable                distance based method of tree

         elements                                   construction ?

    (D) Facilitates transport of solutes            (A) UPGMA


78. The expression of the following                 (B) Maximum parsimony

    genes is essential for the lysogenic            (C) Maximum likelihood

    path of lambda phage when it infects            (D) Bayesian

    E.coli :
                                                81. The term anthesis refers to :

    (A) N, Cro
                                                    (A) Formation of an anther

    (B) CI, CII, CIII                               (B) Opening of a flower

    (C) P, O                                        (C) Development of flower

    (D) att, xis, inf                               (D) Dehiscence of anther

                                           18
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82. If cells of Escherichia coli are grown        85. In mammals, mature functional
     in a medium containing radioactive
                                                       spermatozoa are produced in :
     32P, the 32P would be found in all,

     except :                                          (A) Testis

     (A) DNA                                           (B) Vas deferens

     (B) RNA                                           (C) Epididymis
     (C) ATP
                                                       (D) Seminal vesicle
     (D) Carbohydrates
                                                  86. Isoenzymes are ...................... .
83. The DNA profiling technique which
     demonstrates the similarity between               (A) Oligomeric proteins which have
     different animal species with                           different      physico-chemical
     reference to some specific proteins                     properties and catalyse the
     coding sequences is known as :
                                                             same reaction.
     (A) Zoo blot
                                                       (B) Monomeric proteins which have
     (B) Garden blot
                                                             different      physico-chemical
     (C) Phylogenetic blot
                                                             properties and catalyse the
     (D) Animal profiling
                                                             same reaction.
84. The most important region in the
                                                       (C) Oligomeric proteins which have
     atmosphere for microbial dispersal
     is :                                                    same physico-chemical properties

     (A) Stratosphere                                        and catalyse different reactions

     (B) Troposphere                                   (D) Monomeric proteins which have

     (C) Ionosphere                                          same physico-chemical properties

     (D) Strato-ionosphere                                   and catalyse different reactions.

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87. Molecular clocks located throughout
                                                89. For the reaction A     B at 298 K,
    the body in peripheral tissues are
                                                    the change in enthalpy is – 7
    organized     into   a    coherent,

    hierarchical system by a ‘‘master’’             kJ.mol–1 and the change in entropy

    clock is located :
                                                    is – 25 J.K–1.mol–1. How much is

    (A) In the pineal gland
                                                    the free energy change and whether
    (B) In the adenohypophysis
                                                    the reaction is spontaneous or non-
    (C) In the hypophysis
                                                    spontaneous ?
    (D) In the supra chiasmatic nucleus

        (SCN) of the hypothalamus                   (A)    G = 450 J.mol–1 and reaction

88. Microtubule depolymerizing drug,                      is not spontaneous
    such as colchicine, is expected to :
                                                    (B)    G = 450 J.mol–1 and reaction
    (A) Inhibit mitosis but allow

        cytokinesis                                       is spontaneous


    (B) Inhibit cytokinesis                         (C)    G = 900 J mol–1 and reaction

    (C) Allow      mitosis      beyond
                                                          is spontaneous
        metaphase
                                                    (D)    G = 900 J mol–1 and reaction
    (D) Induce formation of multiple

        contractile rings                                 is not spontaneous

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90. The southern blotting technique
                                              92. Inducible genes are transcribed
    depends on :
                                                  because ..................... .
    (A) Similarities   between    the

        sequences of probe DNA and                (A) The inducer inactivates the
        experimental DNA
                                                        repressor protein
    (B) The molecular mass of proteins

                                                  (B) Repressor binds to the promoter
    (C) The amino acid sequence of a

        protein
                                                  (C) Repressor binds to the operator
    (D) Dissimilarities between the

        RNA and DNA                               (D) Repressor-inducer bind to the


91. A solution is made by mixing 50 ml                  operator

    of 2 M K2HPO4 and 25 ml of 2.0 M
                                              93. Two micron plasmid can be obtained
    KH2PO4. The solution is diluted to

    a final volume of 250 ml. What is             from the following yeast :

    the pH of the final solution ?
                                                  (A) Candida shehatae
    (PK = 6.82)

    (A) 6.82                                      (B) Pichia pastoris

    (B) 7.12
                                                  (C) Schizosaccharomyces pombe
    (C) 6.52

                                                  (D) Saccharomyces cerevisiae
    (D) 7.51

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94. Which of the following is a phagemid          96. What is the applied centrifugal field


    vector ?                                          at a point equivalent to 5 cm from


                                                      the centre of rotation and an angular
    (A) pUC19

                                                      velocity of 3000 rad s–1 ?
    (B) pBR322
                                                      (A) 4.5 × 10–7 cms–2
    (C)   EMBL3
                                                      (B) 5.4 × 10–7 cms–2

    (D) p Bluescript
                                                      (C) 3.4 × 10–7 cms–2

95. Colocalization of two fluorescently
                                                      (D) 6.5 × 10–7 cms–2

    labelled proteins in a cell is usually
                                                  97. Mutation in gene rel A in bacteria
    visualized by :
                                                      results in .................... .

    (A) Phase contrast microscopy
                                                      (A) Relaxed mutants

    (B) Scanning electron microscopy
                                                      (B) Silent mutants


    (C) Confocal microscopy
                                                      (C) Constitutive mutants


    (D) Atomic force microscopy                       (D) Dominant mutants

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98. Glycogen, starch and cellulose are             99. The amino acid that interrupts

    polymers of glucose. Which of the                   -helix conformation in protein by
    following statements are true about
                                                       developing kinks in the structure :
    these polymers ?
                                                       (A) Serine
    (i)   Glycogen and starch are having
                                                       (B) Valine
           1-4 and    1-6 glycosidic bonds.

    (ii) Cellulose is having           1-4             (C) Proline

          glycosidic linkage.                          (D) Leucine

    (iii) All these polymers are giving
                                                   100. Which one-electron carrier protein
          energy for the cells.
                                                       transfers electron from photosystem

    (iv) Glycogen is present in animals
                                                       I to photosystem II and is functionally
          and starch in plants.
                                                       similar    to   cytochrome      C   of
    (v) Amylase enzyme is useful in
                                                       mitochondria ?
          digestion of these polymers.
                                                       (A) Plastoquinone
    (A) (i), (ii) and (v)
                                                       (B) Phycobillin
    (B) (i), (ii) and (iv)

                                                       (C) Phycoerythrin
    (C) (ii), (iii) and (iv)

                                                       (D) Plastocyanin
    (D) (ii), (iii) and (v)
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ROUGH WORK




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Source: Savitribai Phule Pune University. Question paper published by Savitribai Phule Pune University.