SSB Odisha Lecturer 2016: Lecturer recruitment for non-government aided colleges, Biotechnology
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DO NOT OPEN THIS TEST BOOKLET UNTIL YOU ARE ASKED TO DO SO
Subject Code No. : 22 Sl. No. :
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TEST BOOKLET
LECTURERS IN NON.GOVERNMENT
AIDED GOLLEGES
BIOTECHNOLOGY
TimeAllowed:2Hours Maximum Marks : 100
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co - 2t6 (Turn over)
9ol l,
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Unit- | 5. A mitochondrial markerenzyme is :
(BtocHEMlSTRY) (A) Aldolase
1. Enzyme catalyzed hydrolysis of (B) Amylase
proteins produces amino acid of the (C) Succinicdehydrogenase
form:
(D) Pyruvatedehydrogenase
(A) D
(B) DL 6. Which mineral element controls the
(C) Racemic activity of Nitrate reductase ?
(D) L (A) Fe
(B) Mo
2. All of the following compounds are
intermediates of TCA cycle except:
(C) 7r
(A) Pyruvate (D) Ca
(B) Malate 7. Enzyme inhibition caused by a
(C) Oxaloacetate substance resembling substrate
(D) Fumarate molecule is:
3. ldentify the correct molecule which (A) Competitiveinhibition
controls the biosynthesis of proteins (B) Non-competitiveinhibition
in living organisms: (C) Feedbackinhibition
(A) DNA (D) Allostericinhibition
(B) Purines
8. The Michaelis constant, K, is:
(c) RNA
(A) Numericallyequal b 1t2Vr",
(D) Pyrimidines
(B) Dependent on the enzyme
4. Transamtnase activity needs the
concentration
Coenzyme:
(A) ArP (C) lndependentofpH
(B) B6-PO4 (D) Numerically equal to the
(c) FAD substrate concentration that
(D) NAD+ gives half maximal velocity
co -2t6 (2) Contd
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9. Oxidation of which substance in the 13. Which of the following is a substrate
body yields the most calories ?
for aldolase activity in Glycolytic
(A) Glucose Pathway ?
(B) Glycogen
(A) Glyceraldehyde-3-p
(C) Protein
(B) Glucose-6-p
(D) Lipids
(C) Fructose-6-p
10. cr-D-glucose and B-D-glucose are :
(D) Fructose 1, G-bisphosphate
(A) Stereoisomers
(B) Epimers 14. ln protein structure the cr-helix and
(C) Anomers B-sheets are example of :
(D) Keto-aldo pairs (A) Primarystructure
11 . Phenylalanine is the precursor of :
(B) Secondary structure
(A) L-DOPA (C) Iertiary structure
(B) Histamine (D) Quaternarystructure
(C) Tyrosine
15. The number of helices present in a
(D) Throxine
collagen molecule is:
12. An uncoupler of oxidative (A) 1
phosphorylation such as
(B) 2
dinitrophenol:
(c) 3
(A) lnhibits electron transport and
ATP synthesis (D) 4
(B) Allow electron transport to 16. Unsaturated fattyacids is known as:
proceed without ATP synthesis
(A) Non-essentialfattyacids
(C) lnhibits electron transport
without impairment of ATP
(B) Essentialfattyacids
synthesis (C) Cerebrosides
(D) Specially inhibits cytochrome b (D) Phospholipids
co -2t6 (3) (Turn over)
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17. Xanthoproteic test is positive in Unit- Il
proteins containing : (MOLECULAR BTOLOGY AND
(A) Sulphur amino acids GENETTC ENGTNEERTNG)
(B) cr-Amino acids 21. Translation results in the formation
(C) Aromatic amino acids of:
(D) Aliphatic amino acids (A) mRNA
(B) IRNA
18. The half-life of antibody protein is
(C) rRNA
about:
(D) A protein molecule
(A) 4 weeks
(B) 3 weeks 22. ln the'lac operon'concept, which of
the following is a protein ?
(C) 2 weeks
(D) 1 week (A) Operator
(B) Repressor
19. p-Oxidation of odd-carbon fatty acid
(C) lnducer
chain produces:
(D) Vector
(A) SuccinylCoA
23. Another name for reverse
(B) PropionylCoA
transcriptase is :
(C) AcetylCoA
(A) DNA dependent DNA
(D) MalonylCoA
polymerase
20. The general formula of mono- (B) DNA dependent RNA
saccharides is :
polymerase
(A) CH^O
nznn (C) RNA dependent DNA
(B) CrnHrOn polymerase
(c) cnHrorn (D) RNA dependent RNA
(D) CnHrnOrn polymerase
co -2t6 (4) Contd
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24. Nucleic acid show strong absorption 28. The genetic code operates through :
at one of the wavelength : (A) The protein moiety of DNA
(A) 280 nm (B) Cistron of DNA
(B) 220 nm (C) Nucleotide sequence of mRNA
(C) 360 nm (D) fhe anticodons of IRNA
(D) 260 nm 29. Proto-oncogenes are present in :
(A) Oncoviruses
25. Template-directed DNA synthesis
(B) Cancer cells
occurs in allthe following except:
(C) Healthy human cells
(A) The replication fork
(D) Prokaryotes
(B) Polymerase chain reaction
(C) Grovvth of RNAtumorviruses 30. Yeast artificial chromosome can be
used to amplify DNA sequences of
(D) Expression of oncogenes
the size :
26. A Gene is : (A) Upto 10 kb
(A) A single protein molecule (B) Upto 45 kb
(B) A group of chromosomes (C) Upto 100 kb
(C) An instruction for making a (D) Upto 1,000 kb
protein molecule 31. DNA finger printing is based on the
(D) A bit of DNA molecule presence in DNA of :
(A) Constant number of tandem
27. All the following are naturally
repeats
occurring nucleotides except :
(B) Variable number of tandem
(A) Cyclic AMP
repeats
(B) ATP
(C) Non-repetitive sequences in
(c) DNA EACh DNA
(D) lnosine monophosphate (D) Introns in eukaryotic DNA
co -2t6 (5) ( Turn over )
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32. A cosmid is a : (B) They act on double stranded
(A) Large bacterial plasmid DNA
(B) Viral plasmid (C) They recognize palindromic
sequences
(C) Hybrid of plasmid and phage
(D) They always produce sticky
(D) Yeast plasmid ends
33. Optimum temperature of DNA 37 . Suppressor mutations occur in :
polymerase of T. aquaticus is :
(A) Structuralgenes
(A) 3ooc (B) Promoter regions
(B) 37oC (C) Silencerelements
(c) 54oC (D) Anticodons of tRNA
(D) 72oC 38. ln mammalian cell cycle, synthesis of
34. Base sequence of DNA can be DNA occurs during :
determined by: (A) S phase
(A) Maxam-Gilbertmethod (B) G., phase
(B) Folin Method (C) Mitotic phase
(C) Diphenylaminemethod (D) G, phase
(D) Biuriate method 39. The first protein synthesized by
recombinant DNA technology was :
35. A particular protein in a mixture can
(A) Streptokinase
be detected by:
(B) Human grovuth hormone
(A) Southern blotting
(C) Tissue plasminogen activator
(B) Northern blotting (D) Human insulin
(C) Western blotting
40. Lambda phage can be used to clone
(D) Normalblotting DNA fragments of the size :
36. All of the following statements about (A) Upto 3 kilobases
restriction endonucleases are true (B) Upto 20 kilobases
except: (C) Upto 45 kilobases
(A) They are present in bacteria (D) Upto 1,000 kilobases
co -216 (6) Contd
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Unit- lll 45. Which of the following organisms
(MrcRoBroLoGYAN D MMUNOLOGY)
r
lack definite cellwall ?
41. The exact composition of a culture (A) Mycoplasma
medium is known, and it is called : (B) Mucor
(A) Simple medium (C) Mycobacteria
(B) Complex medium (D) Yeast
(C) Defined medium 46. Bacterial cells are active and
(D) Naturalmedium synthesizing new protoplasm. This
42. Which of the following is ionizing stage of groMh is called :
radiation ? (A) Lag phase
(A) U V rays (B) Log phase
(B) IR rays (C) Stationary phase
(C) Gamma rays (D) Death phase
(D) Visible light
47 . Bacteria used organic compound as
43. The growth curve obtained for a theirenergy and electron source are
medium containing two carbon said to be :
sources is called : (A) Phototrophs
(A) Diauxic (B) Autotrophs
(B) Continuous (C) Auxotrophs
(C) Batch (D) Organotrophs
(D) Synchronous
48. The antibodythat is firstformed after
44. Culture medium used forfungus is: infection is:
(A) Sabouraud's medium (A) lgG
(B) Nutrient agar (B) lgM
(C) Nutrient broth (c) lgD
(D) Minimal agar medium (D) lgE
co -2t6 (7) (Turn over )
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49. Antibodies in our body are produced 53. Attenuated, oral poliomyelitis
by: vaccine is :
(A) B-lymphocytes (A) BCG
(B) Measles vaccine
(B) T-lymphocytes
(C) Sabin vaccine
(C) Monocytes (D) TAB vaccine
(D) RBC',s
54. Mountax reaction is used for
50. Active immunity is induced by: detection of :
(A) lnfection (A) rB
(B) Diphtheria
(B) Placentaltransferofantibodies
(C) Cholera
(C) lnjection of antibodies (D) Polio
(D) lnjection of gamma-globulins
55. DuringAIDS, HIV infects :
51. The specificity of an antibody is due (A) CDr lymphocytes
to: (B) CDo lymphocytes
(A) lts valance (C) CDr lymphocytes
(B) The heavy chains (D) B-lymphocytes
(C) The Fc portion of the 56. Chloramphenicolis obtained from :
molecule (A) Streptomyces griseus
(D) The variable portion of the (B) Streptomycesvenezuelae
heayy and light chain (C) Streptococcus mutans
52. Fusion between a plasma cell and a (D) Staphylococcus aureus
tumor cell is called : 57. The etiologic agent of botulism is a :
(A) Myeloma (A) Neurotoxin
(B) Lymphoma (B) Endotoxin
(c) Hybridoma (C) Enterotoxin
(D) Leukemia (D) Exotoxin
co -2t6 (8) Contd.
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58. Which one of the following mineral 62. Which of the following techniques is
elements play an important role in used to separate proteins based
biological nitrogen fixation ? upon differences in their mass ?
(A) Copper (A) lsoelectricfocusing
(B) Magnesium (B) Dialysis
(C) Zinc (C) SDS-gel Electrophoresis
(D) Molybdenum (D) Western blotting
59. Penicillininhibits:
63. ln fermentor, the top portion left
(A) DNA replication without broth is called :
(B) Cell membrane formation
(A) Shaft
(C) Cellwallformation
(B) Head space
(D) Protein synthesis
(C) tmpeller
60. The reaction of soluble antigen with (D) Sparger
antibody is known by :
(A) Precipitation 64. The purification and recovery of the
(B) Flocculation production after fermentation is
(C) Agglutination called:
(D) Complementfixation (A) Upstream process
(B) Downstream process
Unit - lV
(BrorEcHNlQUES AND (C) Surfacefermentation
BIoPROCESS TECHNOLOGY) (D) Submerged fermentation
61. The technique for purification of 65. Microorganisms used for alcohol
proteins that can be made sPecific production :
for a given protein is :
(A) Zymornonas mobilis
(A) Gelfiltration chromatograPhY
(B) Bacillus subtilis
(B) Ion exchange chromatograPhY
(C) Electrophoresis (C) Penicilliumchrysogenum
(D) Affinity chromatograPhY (D) Lactobacillus lactis
co -2t6 (e) (Turn over )
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66. Which method is not used to get 70. Proteins can be visualized directly in
immobilized enzymes ? gels by:
(A) Adsorption (A) Staining them with dye
(B) Encapsulation (B) Using electron microscope
(C) Covalent bonding (C) Measuring the molecularweight
(D) Measuring the distance
(D) Flocculation
between bands
67. Which of the following method is not
71. Fluorescence microscope is a
followed to improve industrialstrains ?
specially modified compound
(A) Protoplastfusion microscope use:
(B) RecombinantDNAtechnique (A) Visible light
(C) Geneticrecombination (B) UV light
(D) De-replication (C) lR radiation
68. The enzyme trypsin is specific for (D) Beam of electron
peptide bonds of : 72. ln Gas chromatography, the
(A) Basic amino acids compounds are separated on the
basis of :
(B) Acidic amino acids
(A) Partition coefficient
(C) Aromatic amino acids
(B) Conductivity
(D) Next to small amino acid
(C) Molarity
residues
(D) Molecularweight
69. The specimen for an electron
73. ln a native PAGE, proteins are
microscope is always:
separated on the basis of :
(A) Stained with dyes
(A) Net negative charge
(B) Sliced into thin section
(B) Net charge and size
(C) Killed (C) Net positive charge and size
(D) Viewed directly (D) Net positive charge
co -2t6 (10) Contd
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74. Which of the following range is (B) Vaned discs
associated with UV spectroscopy ? (C) Marine propeller
(A) 0.8-500 nm (D) Orifice sparger
(B) 100-400 nm 79. Addition of nutrients, acid/alkali to
(C) 380-750 nm fermenter is normally made through :
(D) 0.01-10 nm (A) Steeltube
75. Which among the following is widely (B) Wooden tube
used in bakers yeast production ? (C) Silicone tube
(A) Saccharomyces cerevisiae (D) Rubbertube
(B) Saccharomyces uvarum
80. The hydrogelobtained from natural
(C) Saccharcmycescarlsberyensis polymers used for cell immobilization
(D) Gandida sp. is:
76. ln the citric acid production, the pH (A) Polyacrylamide
to be maintained in the fernmenter (B) Methacrylate
is: (c) Chitin
(A) 7.0 (D) Polyurethane
(B) 5.0 to 6.0
Unit - V
(c) 8.0 to 9.0
(ANIMAL, PLANT AND
(D) 1.0 to 6.0
ENVIRONMENTAL
77 . The precursor of penicillin V is :
BToTECHNOLOGY)
(A) Propionate 81. Which one of the following bacteria
(B) Phenoxyacetic acid has found extensive use in genetic
(C) Anthranilic acid engineering work in plants ?
(D) Dihydroxynovobionicacid (A) Clostridumsepticum
78. Which among the following is NOT (B) Xanthomonas oriza
an impeller ? (C) Bacillus coagulens
(A) Discturbine (D) Agrobacterium tumefaciens
co -2t6 ( 11 ) (Tum over)
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82. The medium used for selection of (B) Bioventing
hybridoma cells is :
(C) Air-sparging
(A) Mac-Conkey medium (D) Biofiltration
(B) MS medium
(C) HAT medium 87. Which hormone pair are required to
(D) PDA medium differentiate callus ?
(A) Auxin and cytokinin
83. Which of the enzymes responsible
for cellulose degradation ? (B) Auxin and ethylene
(A) Endoglucanase (C) Auxin and absiccic acid
(B) Glucosaminidase (D) Ethylene and absiccic acid
(C) Hexokinase 88. Most widely used fusogens in
(D) Xylanase protoplast fusion is :
84. The cells have the capacity to (A) Mannitol
generate different types of cells are (B) Sorbitol
called: (C) Sucrose
(A) Apoptotic cells (D) PEG
(B) Pluripotent cells
(C) Fibroblastic cells 89. Gene knock-out means :
(D) Macrophages (A) lnactivation of specificgene
(B) Entry of specific gene
85. Which of the following bacteria is
called 'oileating bacteria' ?
(C) Purification of specificgene
(A) Pseudomonas putida (D) Hyper-expression of specific
gene
(B) Pseudomonasaeruginosa
(C) Bacillus amyloliquefaciens 90. Diabetes mellitus is due to the
(D) Bacillus subtilis deficiency of :
(A) lnsulin
86. Technique used to add oxygen
directly to a site of contamination in (B) Glucagon
the vadose zone is called : (c) LH
(A) Bioaugmentation (D) FSH
co -2t6 (12) Contd
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91. Metabolic rate in mammals is 95. The plasmids associated with
controlled by: resistance to antibiotics are:
(A) Liver (A) R
(B) Hfr
(C) Thyroid (C) Col
(D) Pituitary
(D) F
92. The hormone known to participate in
96. Antisense RNA molecules have a
metabolism of calcium and
phosphorus is :
sequence to normalRNA
transcripts.
(A) Mineralocorticoids
(B) Calcitonin (A) Complementary
(C) Glucagon (B) Non-complementary
(D) Glucocorticoids (C) Partiallycomplementary
(D) Similar
93. Opines are:
(A) Amino acids derivatives found 97. Which one is effective forthe growth
in normaltissue of shoot apices ?
(B) Amino acids derivatives found (A) Gibberellic acid
in tumor tissue
(B) Auxin
(C) Amino acids derivatives found (C) Cytokinin
in bacterialcells
(D) Ethylene
(D) Amino acids derivatives found
in animalcells 98. Asexual orvegetative propagation of
whole plants using tissue culture
94. The microorganisms leach metals out
of rock ores is : techniques is called :
(A) Pseudomonasaeruginosa (A) Micropropagation
(B) Thiobacillus sp. (B) Organogenesis
(C) Pseudomonas putida (C) Callus culture
(D) Aeromonashydrophila (D) Suspension culture
co -2t6 (13 ) ( Turn over )
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99. Unorganized proliferative mass of 100. Animal that has gained new genetic
cells is called : information from the acquisition of
foreign DNA is a :
(A) Callus
(A) Chimera
(B) Explant
(B) Transgenic animal
(C) Fragments (C) Vector
(D) Totipotent (D) Transferred animal
rrrrllrrtalltlt
co -216 (14) Contd
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SPACE FOR ROUGH WORK co - 2/6 (500) (15) Biotechnology
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