Consider the following statements about enzymatic desizing.
1. The enzyme has an optimum pH and temperature that must be held.
2. Excessive temperature destroys the enzyme.
3. Enzyme activity is unaffected by the water hardness.
Answer: C
Correct answer: 1 and 2 only
Statements 1 and 2 are correct: every enzyme has an optimum pH and temperature, and heating past it denatures the protein irreversibly, ending the activity for good. Statement 3 is wrong - hardness matters, and many bacterial amylases actually require calcium for stability, so the water must be controlled rather than merely supplied.
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